|
KMID : 1059519970410040186
|
|
Journal of the Korean Chemical Society
1997 Volume.41 No. 4 p.186 ~ p.197
|
|
|
Urinary Profiling of Endogeneous Estrogens Using GC/MS
|
|
Lee Seon-Hwa
Yang Yoon-Jung
Kim Tae-Wook
Paeng Ki-Jung
Chung Bong-Chul
|
|
|
Abstract
|
|
|
|
Estrogens, which play an important role in sex hormone and potential inhibitor of cancer cell proliferation were profiled for normal female and male. The nineteen endogeneous estrogens were simultaneously analyzed by selected ion monitoring method of GC/MS. Urinary estrogens were extracted by using Serdolit AD-2 resin, hydrolyzed with β-glucuronidase/arylsulfatase from Helix pomatia, liquid-liquid extraction and quantitatively derivatized by MSTFA/TMSCl mixture in order to be detected on the GC/MS. The good quality-control data were obtained through the precision and accuracy test and the recovery range of them was 80.97∼97.81%. The Korean reference values of urinary estrogens were established and differences were found in normal female compared with normal male.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|